Radiotelemetry tracking of adult muskellunge (Esox masquinongy) was used to locate muskellunge spawning and nursery habitats in the Thousand Islands region of the St. Lawrence River and to describe post-spawning movements. From 1984 to 1989, 47 adult muskellunge were radio tagged during the spawning season at 22 sites. Radio-tagged fish were located at least once daily during the spawning period. Capture sites of adult muskellunge and areas frequented by tagged muskellunge during the spawning period were subsequently seined to document the presence or absence of young-of-the-year (YOY) muskellunge. YOY muskellunge were collected at 13 of the 22 adult capture sites and also at an additional 14 sites frequented by radio tagged adults. Post-spawning muskellunge generally either migrated upstream to Lake Ontario, remained in the vicinity of their respective spawning site or moved into deep water where they could not be located. Radio transmitters that functioned into the following season revealed a high degree of reproductive homing.